Abstract

This disclosure describes the molecular architecture and primary amino acid sequence of a recombinant fusion protein comprising a canine-derived Fms-like tyrosine kinase 3 ligand (FLT3L) extracellular domain and a collagen-binding domain (CBD). The construct is designed for expression in mammalian systems and includes a signal peptide for secretion, a flexible linker to preserve functional domain activity, and a C-terminal purification tag.

The fusion protein is configured to associate with collagen-rich extracellular matrices through the CBD moiety, enabling localized presentation of FLT3L within tissue environments. FLT3L is a hematopoietic growth factor involved in the expansion and differentiation of dendritic cell populations, including conventional type 1 dendritic cells (cDC1), which are implicated in antigen presentation and immune activation.

The disclosed sequence represents a canine-specific implementation of a collagen-binding cytokine fusion protein and is intended for use in veterinary biomedical research and therapeutic development. Expression, purification, and formulation approaches are described to support reproducible production of the construct.

This disclosure establishes the sequence and structural configuration of the FLT3L-CBD fusion protein as publicly accessible prior art and encompasses functional variants and encoded nucleic acid constructs consistent with the disclosed architecture.

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Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

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