Abstract
GCI #270 v4.1 is an open‑hardware, home‑deployable diagnostic for Mycoplasma pneumoniae and S. pneumoniae (walking pneumonia). Uses extraction‑free one‑pot Cas12a‑LAMP‑CRISPR (1 copy/µL LoD, 98–100% sensitivity/specificity) in a Vortex‑HT microfluidic disc with sonication lysis, photo‑gated activation, and PCM incubation (10–20 min total). 16‑channel multiplex, smartphone AI readout. 3–6x faster, 20–80x cheaper than Alethia/BioFire RP2.1. TRL 8–9; validated chemistry (CHAMP, ERA‑Cas12a trials). BOM $1.20/test; 4‑step workflow. CERN‑OHL‑P licensed for global manufacture. Requires wet‑lab validation for clinical deployment.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Recommended Citation
Caldwell, Michael Victor Mr., "GCI #270 v4.1: Home Dual Pneumonia Detector (Vortex‑HT Cas12a LAMP‑CRISPR)", Technical Disclosure Commons, (March 25, 2026)
https://www.tdcommons.org/dpubs_series/9615