Abstract
Many point of care devices use enzymatic assays to quantify the analyte of interest. Enzyme efficiency can be strongly temperature dependent. Therefore, it is important to correct for temperature effects during the analysis of experimental results. This disclosure describes techniques to correct for temperature effects in enzymatic assaying by using thermochromic liquid crystals in parallel with the enzymatic assay to track real time temperature variation at the assay location. Temperature can thus be tracked at the precise assay location, rather than at some nearby point. By thus directly and empirically measuring temperature at the location that matters, assumptions about the distribution of temperature can be eliminated. Accurate knowledge of the temperature at the exact point of assay is thus obtained, improving accuracy in situations where relatively small variations in temperature can cause significant variations in experimental results.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Recommended Citation
Watkins, Herschel and Richards, Pete, "Temperature Correction in Optical Enzymatic Assays Using Thermochromic Liquid Crystals", Technical Disclosure Commons, (December 27, 2023)
https://www.tdcommons.org/dpubs_series/6547